Comparison of immunization methods for producing reference adenovirus antisera in horses.

Prognosis of Hepatic Amyloidosis Status and Insufficiency in Snake Antivenom Producing Horses

Hyperimmunization of horses with specific antigens to harvest antisera is associated with high incidence of liver amyloidosis. The histopathologic examination is the gold standard method for diagnosis of the hepatic amyloidosis. The aim of the present study was to determine whether the clinical manifestations and serum values of appropriate chemical constituents due to liver injury, provide the...

Recent Advances in Immunization of Horses Against African Horsesickness

Reference equine antisera to 33 human adenovirus types: homologous and heterologous titers.

Equine antisera to human adenovirus types 1 to 33 were prepared and evaluated by hemagglutination-inhibition and serum neutralization tests. Detailed data on the potency and purity of the immunizing antigens were tabulated as one means of evaluating the antisera. Most of the 52 hemagglutination-inhibition and 25 serum neutralization major or minor heterotypic responses among the equine antisera...

P-73: Effect of Donor Age on The Expression Stability of GAPDH as A ReferenceGene for Gene Expression Analysis ofEquine Adipose-Derived Mesenchymal Stem Cells

Background: Adipose tissue is a main source for isolation of equine mesenchymal stem cells (MSCs) at different ages. It seems that characteristics of adipose-derived MSCs especially gene expression profile are changing along with age increase. A proper reference gene is required for normalizing data in gene expression analysis by qRT-PCR. This study aimed to evaluate whether GAPDH has a stable ...

Development of neuraminidase subtype-specific reference antisera by recombinant protein expressed in baculovirus.

Outbreaks of avian influenza A virus infection, particularly the H5N1 strains that have affected birds and some humans for the past 15 years, have highlighted the need for increased surveillance and disease control. Such measures require diagnostic tests to detect and characterize the different subtypes of influenza virus. In the current study, a simple method for producing reference avian infl...